技术资料：https://iscms.westlake.edu.cn/technique/SOP-Chirascan-Plus-V100.pdf

样品要求：以吸光度在0.2-2.0AU 之间来准备样品。
   1. 蛋白质（多肽）溶液：分子量低于10000时，建议1mg/mL；大于10000时，建议0.5mg/mL。溶液里不能含有DMF或DMSO。如使用除纯水以外的溶剂，需随样品提供少量该溶剂。
   2. 核酸和其它有机小分子以及配位化合物：用1mm或10mm光程的比色皿测量时吸收值低于2.0，并且随样品提供UV-Vis图谱。
   3. 固体：可以制成薄膜，或者溶剂挥发后能够粘附在石英玻璃片上，或者制成KCl或KBr压片，或者有足够的量盖住13mm直径的石英片用于漫反射测试。

Instrument No.  20103104-9004                            Specification  

Manufacture     Applied Photophysics Ltd             Model                        Chirascan V100

Made in            United States                               Classification No          010411

Location           4#220                                          Manufacture date         11/09/2019

Purchase Date  15/7/2019                                     Internet access time     1/11/2019

Main Technical specifications 

1. Light source: 150W xenon lamp (UV-visible), halogen lamp (NIR), mercury xenon lamp (applicable to stopped-flow unit),

2. Detector: 170nm-1200nm, APD detector; 850-1700nm, InGaAs detector.

3. Wavelength accuracy: ±0.1nm (160 ~ 500nm) ±0.5nm (500 ~ 1700nm)

4. Adjustable range of spectral bandwidth: 0 ~ >25nm.

5. Stray light: less than 3ppm (200nm).

6. CD noise level:

0.050 m @ 175 nm

0.020 m @ 180 nm

0.030 m @ 200 nm

0.03 mdeg @ 750 nm

0.05 mdeg @ 1000 nm

(detection conditions: 2 second oversampling, 1 nm bandwidth)

7. Measurement range of CD: not less than ±9000 m°

8. Scanning speed: 1-10,000 nm/min

9. Electronic temperature control system (including cyclic bath and magnetic stirring) : -40℃-150℃, accuracy: ± 0.1℃

10. Spectral scanning together with temperature continuous and stepped ramping to study thermodynamic process (Tm, Δ H)

11. Fluorescence polarization/anisotropy detection module: the wavelength range not narrower than 185-850nm.

12. Fast kinetics stopped flow device

Mixing mode: contains two or three samples mixing;

Quench stopped flow device: samples can be recovered for further analysis;

Minimum dead time: better than 1.1 ms (20-micrometer sample, water-water system as the standard);

13. Solid sample transmission measurement accessory

14. Solid sample reflection integrating sphere accessory.

15. Array fluorescent CCD detection.

Main functions and features

Functions:

1. Study the secondary and tertiary structures of biological macromolecules;

2. Study the thermal stability of biological macromolecules and organic polymers;

3. Study on chiral stereoscopic structures of various chiral compounds;

4. Study the millisecond rapid-reaction kinetics mechanism of intermolecular interaction (reaction speed, intermediate transition state existing for a short time), etc.

Features:

Effective detectable spectral range: 175-1700nm;

Sample types: liquid, solid (tablet, film, coating, powder), gas samples, please inquire for details.

Working mode: Static, Kinetics.

A variety of light sources are available.

Services available:

1. Provide static circular dichroic CD detection;

2. Provide reaction kinetics analysis;

3. Provide the use of the instrument;

Informations:

Sample requirements: prepare samples with an absorbance between 0.2 and 2.0AU.

1. Protein (polypeptide) solution: 1mg/mL is recommended when the molecular weight is less than 10000; if the molecular weight exceed 10000, 0.5mg/mL is recommended. Sample solution can’t include DMF or DMSO. If use non-water solvent, a small amount of this solvent must be supplied as “Blank”.

2. Nucleic acids, other small organic molecules and coordination compounds: the absorbance value should be less than 2.0 measured in 1mm or 10mm cuvette, and UV-VIS spectra should be provided with the CD spectra of samples.

3. Solid: it can be made into a thin film, or it can stick to the quartz glass after solvent evaporation, or it can be pressed into KCl or KBr tablet, or it is enough to covers the 13mm diameter quartz sheet for diffuse reflection test.