| 主要规格及技术指标 |
1、激光器系统
1.1 固体激光器：405nm；
1.2 脉冲激光器：
脉冲白激光器: 在485nm-685nm范围内，步进精度≤1nm，自由选择激发谱线进行成像，同时输出脉冲激光谱线≥8条。
1.3 高效多色分光系統：
配备声光调制晶体分光系统，由声光器件控制，无需滤光片和机械切换，最多可同时调节出8根激发谱线，每根激发谱线的强度均可独立调节，调节精度0.01%；
1.4 激光器开闭和电压调节由计算机的激光共聚焦扫描软件系统控制，与整个系统偶合程度高，电噪声小，安全，并有良好的激光管寿命保护装置。
1. Laser system
1.1 Solid-state laser: 405nm;
1.2 Pulse laser:
Pulsed white laser: In the range of 485nm-685nm, the step accuracy is ≤1nm, the excitation spectrum can be freely selected for imaging, and at the same time, ≥8 pulsed laser spectrum lines can be output.
1.3 High-efficiency multi-color spectroscopic system:
Equipped with an acousto-optic modulation crystal spectroscopic system, controlled by an acousto-optic device, without filters and mechanical switching, up to 8 excitation lines can be adjusted at the same time, and the intensity of each excitation line can be adjusted independently, with an adjustment accuracy of 0.01%;
1.4 The laser on-off and voltage adjustment are controlled by the laser confocal scanning software system of the computer, which has a high degree of coupling with the entire system, low electrical noise, safety, and a good laser tube life protection device.
2、扫描检测系统
2.1 激光扫描组件与所接显微镜一体化设计，一体化像差及色差校正，以保证高质量，高分辨率成像。软件对硬件的有效控制，使系统有优异的稳定性及可维护性，光纤耦合和镜耦合可接低功率激光器；
2.2 能够进行X、Y、Z、T、λ（发射光谱扫描）、Λ（激发光谱扫描）、θ（旋转角度）、I（光强度）、A（区域）等多维组合扫描，可实现点扫描、线扫描、曲线扫描、区域扫描、光谱波长扫描等。可最多同时进行5个荧光信号外加1个透射光的采集；
2.3 光谱检测装置:高效率棱镜分光系统,配备发射光调节步进1nm,连续检测荧光波长范围410～850nm；
2.4 可见光专用光学扫描部件，波长校正范围不少于410～850nm；
2.5 最大扫描视场对角线≥22mm；
2.6高分辨率扫描振镜：扫描速度≥10帧/秒（512×512分辨率）；双向扫描速度不少于5400线/秒；扫描速度调节步进1Hz，不少于100档扫描速度调节；光学扫描放大0.75X～48X，连续可调；
2.7单视野最大扫描分辨率≥8000×8000；
2.8高分辨率扫描头具有线性和正弦两种扫描方式，线性扫描提供非对称的扫描方式，确保每一个像素点具有相同的扫描时间，保证定量研究的准确度，在400Hz的单向扫描速度下，单个像素点的驻留时间≥3微秒；
2.9内置超高灵敏自由可调光谱型荧光检测器4个，每个荧光检测器都可做全光谱自由扫描和成像；检测器均为超高灵敏度的硅基阵列式雪崩型二极管混合型检测器，在500nm处量子探测效率≥55%；
2.10系统需配备不少于4个具备时间分辨成像功能检测器，采样频率≥10.3G Hz, 可在0-12ns间调节检测时间，实现荧光寿命分析；
2.11透射光明场检测器：1个PMT检测器。
2. Scanning detection system
2.1 The integrated design of the laser scanning component and the connected microscope, integrated aberration and chromatic aberration correction, to ensure high-quality, high-resolution imaging. The effective control of the software to the hardware makes the system have excellent stability and maintainability, and the fiber coupling and mirror coupling can be connected to low-power lasers;
2.2 It can perform X, Y, Z, T, λ (emission spectrum scanning), Λ (excitation spectrum scanning), θ (rotation angle), I (light intensity), A (area) and other multi-dimensional combined scanning, and point scanning can be realized , line scan, curve scan, area scan, spectral wavelength scan, etc. A maximum of 5 fluorescent signals plus 1 transmitted light can be collected at the same time;
2.3 Spectrum detection device: high-efficiency prism spectroscopic system, equipped with emission light adjustment step 1nm, continuous detection of fluorescence wavelength range 410-850nm;
2.4 Optical scanning components dedicated to visible light, with a wavelength correction range of not less than 410-850nm;
2.5 The diagonal line of the maximum scanning field of view is ≥22mm;
2.6 High-resolution scanning galvanometer: scanning speed ≥ 10 frames per second (512×512 resolution); bidirectional scanning speed not less than 5400 lines per second; scanning speed adjustment step 1Hz, no less than 100 scan speed adjustments; Optical scanning magnification 0.75X ~ 48X, continuously adjustable;
2.7 The maximum scanning resolution of a single field of view is ≥8000×8000;
2.8 The high-resolution scanning head has two scanning methods: linear and sinusoidal. Linear scanning provides an asymmetrical scanning method to ensure that each pixel has the same scanning time and ensure the accuracy of quantitative research. At a unidirectional scanning speed of 400Hz , the dwell time of a single pixel point is ≥3 microseconds;
2.9 There are 4 built-in ultra-high-sensitivity freely adjustable spectrum fluorescence detectors, each of which can do full-spectrum free scanning and imaging; the detectors are ultra-high-sensitivity silicon-based array avalanche diode hybrid detectors, Quantum detection efficiency ≥55% at 500nm;
2.10 The system needs to be equipped with no less than 4 detectors with time-resolved imaging function, the sampling frequency is ≥10.3G Hz, and the detection time can be adjusted between 0-12ns to realize the fluorescence lifetime analysis;
2.11 Transmission bright field detector: 1 PMT detector.
3、荧光寿命传感成像分析系统
3.1系统需配备不少于4个单分子级别检测器，在500nm处量子探测效率不少于55%，光子计数速度不少于160 million counts/s，具有0、1、2三个维度来精确检测光子。额外配备外部制冷设备；
3.2使用內置脉冲激光器作为激发光源,详见第1.2条技术要求；
3.3时间分辨率：≤97ps；
3.4计数速率：≥160 million counts/s；
3.5系统可以基于荧光寿命信息去除反射光和样品自发荧光，提高图像反差，同时可以截取两个光子到达时间窗口来获取有效信号；
3.6 系统可以通过测量平均荧光到达检测器的时间，得到荧光寿命信息，使成像的结果可以直接包含平均荧光到达信息，从而使一次成像得到多种数据，可用于组织自发荧光成像；
3.7 系统可以通过测量平均荧光到达检测器的时间，利用荧光寿命信息直接对染料拆分,能够有效区分光谱重叠而荧光寿命不同的荧光组分。
3. Fluorescence lifetime sensing imaging analysis system
3.1 The system needs to be equipped with no less than 4 single-molecule level detectors, the quantum detection efficiency at 500nm is no less than 55%, the photon counting speed is no less than 160 million counts/s, and it has three dimensions of 0, 1, and 2 to accurately Detect photons. Additional external cooling equipment;
3.2 Use the built-in pulsed laser as the excitation light source, see the technical requirements in Article 1.2 for details;
3.3 Time resolution: ≤97ps;
3.4 Counting rate: ≥160 million counts/s;
3.5 The system can remove reflected light and sample autofluorescence based on fluorescence lifetime information, improve image contrast, and can intercept two photon arrival time windows to obtain effective signals;
3.6 The system can obtain fluorescence lifetime information by measuring the time when the average fluorescence reaches the detector, so that the imaging result can directly include the average fluorescence arrival information, so that a variety of data can be obtained in one imaging, which can be used for tissue autofluorescence imaging;
3.7 The system can directly split the dyes by measuring the average fluorescence arrival time and using the fluorescence lifetime information, which can effectively distinguish the fluorescent components with overlapping spectra and different fluorescence lifetimes.
4、激光共聚焦高分辨率系统
4.1成像分辨率：XY方向≤120nm，Z方向≤250nm，在高分辨率模式下支持在线大视野拼图；
4.2检测器为硅基阵列式雪崩型二极管混合型检测器，可进行光谱式成像，光谱检测范围410-850nm；
4.3同一个实验中可实现蓝、绿、红、红外4种颜色的高分辨率成像，通过线粒体膜蛋白标记，在XY层面能观察到线粒体为中空的腔体结构；也能保证活细胞实验中，在线同时4色高分辨率成像；
4.4所有适合配置激光器激发的荧光样品均可进行高分辨率成像，无需选择特殊荧光抗体及试剂；
4.5同一样品具有与激光共聚焦相同的成像深度；
4.6高分辨率成像为线性成像，所有高分辨率成像均可用作定量分析，如荧光强度分析、FRAP分析等。
4. Laser confocal high-resolution system
4.1 Imaging resolution: XY direction ≤ 120nm, Z direction ≤ 250nm, supports online large field of view mosaic in high resolution mode;
4.2 The detector is a silicon-based array avalanche diode hybrid detector, which can perform spectral imaging, and the spectral detection range is 410-850nm;
4.3 In the same experiment, high-resolution imaging of blue, green, red, and infrared colors can be realized. Through mitochondrial membrane protein labeling, the hollow cavity structure of mitochondria can be observed at the XY level; , online simultaneous 4-color high-resolution imaging;
4.4 All fluorescent samples suitable for laser excitation can be imaged at high resolution without the need to select special fluorescent antibodies and reagents;
4.5 The same sample has the same imaging depth as confocal laser;
4.6 High-resolution imaging is linear imaging, and all high-resolution imaging can be used for quantitative analysis, such as fluorescence intensity analysis, FRAP analysis, etc.
5、光学显微镜系统
5.1研究级全自动倒置显微镜：具备明场、荧光、微分干涉观察功能。显微镜控制可通过彩色触摸屏、遥控器、机身按钮、共聚焦软件来控制；
5.2显微镜透射光源：LED光源，色温4500K；
5.3镜体电动Z轴调焦，调焦行程≥12mm；
5.4电动聚光镜，电动照明光轴，电动调节透射光和荧光的孔径光阑和视场光阑；
5.5全自动DIC调节，当改变物镜倍数时，该倍数DIC所需要的起偏器，检偏器，物镜棱镜，聚光镜自动转移到光路中，可直接在成像软件中直接调节棱镜角度，改变DIC浮雕效果；
5.6长寿命荧光光源，光纤导光，对镜体无热辐射；
5.7电动6孔荧光滤色块转盘，自动荧光强度管理系统，5档荧光光强调节，12个可调视场光阑；
5.8荧光激发块：配备紫外激发滤块、蓝色激发滤块和绿色激发滤块；
5.9宽视野双目观察筒，倾角45°，瞳距调节55-75mm，视场数25mm；
5.10宽视野平场目镜10倍，视场数25mm，屈光度可补偿调节；
5.11物镜：
5.11.1 10倍共聚焦专用干镜，数值孔径N.A.≥0.4；
5.11.2 20倍共聚焦专用干镜，数值孔径N.A.≥0.75；
5.11.3 40倍共聚焦专用干镜，数值孔径N.A.≥0.95；
5.11.4 63倍共聚焦专用油镜，数值孔径N.A.≥1.40；
5.12电动扫描式载物台，行程83mm×127mm，配备通用样品夹，适合直径24-68㎜的培养皿，适合长度≤120mm的玻片；最小步进：0.02μm，重复精度：1μm，外接控制器，可电脑控制或控制器移动载物台；
5.13 配备硬件对焦系统，利用内置远红外LED光源对样本快速和稳定长时间对焦；
5.14 配备活细胞培养系统，兼容35/50/60mm培养皿和多孔板等多种培养容器，具有样品测温和样品温度反馈功能，CO2浓度可控范围5%-20%，温度可控范围10-50℃。
5. Optical microscope system
5.1 Research-level automatic inverted microscope: with bright field, fluorescence, differential interference observation functions. Microscope control can be controlled through color touch screen, remote control, body buttons, and confocal software;
5.2 Microscope transmission light source: LED light source, color temperature 4500K;
5.3 Mirror body electric Z-axis focusing, focusing stroke ≥ 12mm;
5.4 Motorized condenser, motorized illumination optical axis, motorized adjustment of aperture diaphragm and field diaphragm for transmitted light and fluorescence;
5.5 Fully automatic DIC adjustment, when changing the multiple of the objective lens, the polarizer, analyzer, objective prism, and condenser lens required by the multiple DIC are automatically transferred to the optical path, and the prism angle can be directly adjusted in the imaging software to change the DIC relief effect;
5.6 Long-life fluorescent light source, optical fiber light guide, no heat radiation to the mirror body;
5.7 Motorized 6-hole fluorescence filter turntable, automatic fluorescence intensity management system, 5 levels of fluorescence intensity adjustment, 12 adjustable field of view diaphragms;
5.8 Fluorescence excitation block: equipped with ultraviolet excitation filter block, blue excitation filter block and green excitation filter block;
5.9 Wide-field binocular observation tube, with an inclination angle of 45°, an interpupillary distance adjustment of 55-75mm, and a field of view of 25mm;
5.10 wide field of view flat field eyepiece 10 times, the number of field of view is 25mm, the diopter can be adjusted by compensation;
5.11 Objective lens:
5.11.1 10x confocal dedicated dry lens, numerical aperture N.A.≥0.4;
5.11.2 20x confocal dedicated dry lens, numerical aperture N.A.≥0.75;
5.11.3 40 times confocal dedicated dry lens, numerical aperture N.A.≥0.95;
5.11.4 63 times confocal special oil lens, numerical aperture N.A.≥1.40;
5.12 Motorized scanning stage, stroke 83mm×127mm, equipped with universal sample holder, suitable for petri dishes with a diameter of 24-68mm, suitable for slides with a length of ≤120mm; minimum step: 0.02μm, repeatability: 1μm, external control The device can be controlled by computer or the controller can move the stage;
5.13 Equipped with a hardware focusing system, using the built-in far-infrared LED light source to focus on the sample quickly and stably for a long time;
5.14 Equipped with living cell culture system, compatible with various culture vessels such as 35/50/60mm culture dish and multi-well plate, with sample temperature measurement and sample temperature feedback function, CO2 concentration controllable range 5%-20%, temperature controllable range 10 -50°C.
6、计算机工作站
6.1 高配置品牌专业工作站：6核CPU(Intel Xeon W-2133)，内存≥64GB，高性能CUDA GPU(Nvidia Quadro RTX 5000)显卡3072个内核，显存≥16GB，液晶真彩21:9高清显示屏(1个)≥37.5寸，显示屏分辨率≥3840 x 1600，双固态硬盘（256GB+1TB），硬盘≥6TB，16x DVD+/- RW刻录，Windows 10 Professional (64 位)操作系统。
6. Computer workstation
6.1 High-configuration brand professional workstation: 6-core CPU (Intel Xeon W-2133), memory ≥ 64GB, high-performance CUDA GPU (Nvidia Quadro RTX 5000) graphics card with 3072 cores, video memory ≥ 16GB, LCD true color 21:9 high-definition display (1) ≥37.5 inches, display resolution ≥3840 x 1600, dual solid-state drives (256GB+1TB), hard drives ≥6TB, 16x DVD+/- RW burner, Windows 10 Professional (64-bit) operating system.
7、软件系统
7.1软件建立在Windows 10系统上，使用先进程序语言，程序执行效率高，快，稳定。整个系统程序，包括控制，检测、分析功能设计合理，操作界面友好，操作简便；
7.2控制硬件的功能：控制电动显微镜、选择激光波长、调节激光强度、拍摄2-5维图像、选择光谱拍摄范围、成像分辨率、实验条件实时记录、一键式恢复等；
7.3可进行时间记录，可设置时间循环，具有自动聚焦功能，具有荧光亮度校正、补偿功能（在Z轴方向上补偿荧光亮度的变化），可在软件中对DIC效果进行调节；
7.4三维重构软件：具有多种三维重构渲染方式，包括最大强度投影、透明、深度标识和阴影投影等方式，允许xy、xz、yz任意角度进行切面观察，可对重构图进行任意角度旋转、平移、放大和缩小，可对每个荧光通道的强度、灰阶、伽马值及透明度进行独立调节，可根据用户需要对不同荧光通道进行颜色分割显示，可将复杂的3D重构效果导出成电影文件；
7.5光谱拆分软件：能对样品发射荧光进行从400-800nm光谱扫描，可实现在线光谱拆分和扫描后光谱拆分；
7.6共定位分析软件：通过散点图法对双色荧光数据进行共定位分析，可分别对每个通道的背景及阈值进行调节，得出共定位百分比及皮尔森相关系数等统计数据，数据可导出至Excel表格；
7.7图像调节亮度、对比度，单个通道分别调节或多个通道同时调节；
7.8图像处理：旋转、裁剪、多种滤镜、添加标尺、箭头、文字等；
7.9多功能全标本导航，全标本拼图。能进行自定义ROI形状的拼图，能拼接出长条形或圆形的大图，节省不必需的区域成像，加快拼图速度。能指定不同ROI区域使用不同的物镜进行拼图。能一次性批量化扫描多个标本多个ROI拼图；
7.10能进行全片无缝拼图扫描，带聚焦地形图功能，能适应标本高低不同的焦面进行多焦点自动对焦及拼图。用户能自定义多个不同的焦点。能结合电动Z轴进行三维拼图，拼接结果能根据需求进行大图三维重建、大图三维叠加；
7.11针对不同的耗材/器皿，软件能调出并校正6，12，24，48，96孔板的分布图，以及35mm/50mm/60mm培养皿的分布图，2、4、8、16孔等多种chamber coverglass培养小室的分布图等；通过点击软件界面中每个孔，能预览、扫描、定义对应的该孔的细胞图像。能进行多孔板分别每孔的自动对焦、焦点记忆与重返。能进行多孔板分别每孔的拼图、多孔多视野成像;
7.12三维图像测量分析：包含三维交互式测量和三维自动测量，可得出三维空间距离、体积、表面积、角度等数据。三维自动测量具有流程向导功能，包含从图像前处理、阈值调整、生成3D二进制掩膜、测量到生成报告一系列流程。用户定义的分析流程可存储并用于其他数据，分析结果可与实验一起保存，或与曲线和图像一起输出为Excel报告，用于记录或进一步分析，可一次进行多个实验数据集的批处理分析。
7.13具备快捷简便的成像设置导航系统，拖放式设置共聚焦成像，界面简单，一目了然，提高共聚焦的使用效率。
7. Software system
7.1 The software is built on the Windows 10 system, using advanced programming language, the program execution efficiency is high, fast and stable. The entire system program, including control, detection and analysis functions, is designed reasonably, with friendly operation interface and easy operation;
7.2 Control hardware functions: control motorized microscope, select laser wavelength, adjust laser intensity, take 2-5D images, select spectral shooting range, imaging resolution, real-time recording of experimental conditions, one-button recovery, etc.;
7.3 It can record time, set time cycle, have automatic focus function, have fluorescence brightness correction and compensation function (compensate the change of fluorescence brightness in the Z-axis direction), and can adjust the DIC effect in the software;
7.4 Three-dimensional reconstruction software: It has a variety of three-dimensional reconstruction rendering methods, including maximum intensity projection, transparency, depth marking and shadow projection, etc., allowing cross-section observation at any angle of xy, xz, yz, and any angle for reconstruction Rotate, translate, zoom in and zoom out, independently adjust the intensity, gray scale, gamma value and transparency of each fluorescent channel, and display the color segmentation of different fluorescent channels according to user needs, and complex 3D reconstruction effects Export as a movie file;
7.5 Spectrum splitting software: It can scan the spectrum of the sample emission fluorescence from 400-800nm, and can realize online spectrum splitting and spectrum splitting after scanning;
7.6 Co-localization analysis software: Co-localization analysis is performed on two-color fluorescence data by scatter diagram method, the background and threshold of each channel can be adjusted separately, and statistical data such as co-localization percentage and Pearson correlation coefficient can be obtained, and the data can be exported to an Excel table;
7.7 Adjust the brightness and contrast of the image, adjust a single channel separately or adjust multiple channels at the same time;
7.8 Image processing: rotation, cropping, multiple filters, adding scales, arrows, text, etc.;
7.9 Multi-functional full-specimen navigation, full-specimen puzzle. It can customize the puzzle of ROI shape, and can stitch together a long or circular large image, saving unnecessary area imaging and speeding up the puzzle. Different ROI areas can be specified to use different objective lenses for collage. It can batch scan multiple specimens and multiple ROI puzzles at one time;
7.10 It can scan the whole film seamless mosaic, with the function of focusing topographic map, and can adapt to the focal plane of different heights of specimens for multi-focus auto-focus and mosaic. Users can customize multiple different focal points. Combined with the electric Z-axis for 3D mosaic, the mosaic result can be used for large-scale 3D reconstruction and large-scale 3D superposition according to requirements;
7.11 For different consumables/vessels, the software can recall and correct the distribution maps of 6, 12, 24, 48, and 96-well plates, and the distribution maps of 35mm/50mm/60mm culture dishes, 2, 4, 8, 16 holes, etc. Distribution diagrams of various chamber coverglass culture chambers, etc.; by clicking each well in the software interface, you can preview, scan, and define the corresponding cell image of the well. It can perform automatic focus, focus memory and return of each well of the multi-well plate. Capable of mosaicing each well of the multi-well plate, multi-well multi-field imaging;
7.12 Three-dimensional image measurement and analysis: including three-dimensional interactive measurement and three-dimensional automatic measurement, which can obtain three-dimensional space distance, volume, surface area, angle and other data. The 3D automatic measurement has a process wizard function, including a series of processes from image preprocessing, threshold adjustment, 3D binary mask generation, measurement to report generation. The user-defined analysis process can be stored and used for other data, and the analysis results can be saved together with the experiment, or output as an Excel report together with the curve and image for recording or further analysis, and batch analysis of multiple experimental data sets can be performed at one time .
7.13 has a quick and easy imaging setting navigation system, drag and drop setting confocal imaging, simple interface, clear at a glance, and improve the efficiency of confocal use."
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